f5 multi-mode microplate. Each kit was used on the same day that its reagents were reconstituted. f5 multi-mode microplate

 
 Each kit was used on the same day that its reagents were reconstitutedf5 multi-mode microplate  Absorbance was measured at 450 nm using a FilterMAx F5 Multi-Mode Microplate Reader and EX2 filter after a 2-hour incubation at 37°C with 10 μL of CCK-8 per 100 μL of media

Coculture experiment. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. ABS Test results. Set up plate with cells, reagent and test compounds. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. Molecular Devices’ FilterMaxTM F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. 3 Pages. 00. A microplate consists of small wells wherein separate reactions take place. The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). PerkinElmer; Revvity; Customer Transformation Hub. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se The method is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) under conditions of high temperature and acidity that generates a fluorochrome, which is quantified by fluorimetry (F5 Multi-Mode Microplate Reader), with excitation at 515 nm and emission at 553 nm. The amount of the colorimetric product was monitored continuously for 120 min. Fold changes were calculated as the area under the curve of stimulated HKLs relative to unstimulated HKLs (RPMI without zymosan). The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536. , Sunnyvale, CA, USA) and normalized to the absorbance in the. Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). Table of Contents. 40266 Email: [email protected]™ F3 and F5 Multimode Microplate Readers, Molecular Devices. SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. Samples were considered positive if the average sample absorbance was greater than twice the mean absorbance of healthy. Page: 1/4NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. FilterMax F3 & F5 Microplate Readers. 2 Pages. Application:. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Protein quantification was carried out using the Pierce™ BCA Protein Assay Kit (Thermo Fisher) and a FilterMax F5 Multi-Mode Microplate Reader (San Jose, CA, United SA). With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. View specifications, prices, citations, reviews, and more. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. 2 μM ATP standard was. 3 software (Molecular Devices). Chemiluminescence emission was measured in real time (every 2 min for 120 min) with a FilterMax F5 Multi-Mode Microplate Reader at 27°C and expressed as area under the curve, as previously described . Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 667 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. 00 USD. , Sunnyvale, CA, USA). Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. The measurement can be performed using a variety of detection technologies: the. Instrument Setup: 1. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceF3/F5 Multi-Mode Microplate Readers. For 3D cell proliferation assay, (high concentrations) in 3D cellular proliferation assay, 20, 100 or 500 µg/ ml MG were used. Accurate measurements are ensured by automatic lamp calibration prior to each reading. Fred A. ScanLater Western Blot Detection System. 3 Pages. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe parasite viability was determined by resazurin assay (0. 713579). com A. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by normalizing to total protein. The following document is intended to demonstrate setup of this instrument. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seEye lysates were developed using a Glucose Colorimetric Assay Kit (Sigma, Louis, MO, USA) and measured by a FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 570 nm. Jurkat cells were plated in a 12-well dish at a density of 8 x 10 5 cells in 1 mL media. In . Synergy HTX and Synergy LX. 2 Temperature and cooling rate control. SpectraMax iD5. 3 Pages. 4 Pages. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. 50/50. The absorbance was measured at 450 nm with a FilterMax F3 & F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). This microplate reader also features BioTek’s Hybrid Technology, which. Based on a rugged, simple design, the VMax Absorbance Microplate Reader offers a mean time between failure (MTBF) of 20,000 hours with 97. 00. Seoul, South Korea. BMG Labtech - FLUOSTAR OMEGA. Transfluor Technology. 4 Pages. book Page 1 Tuesday, September 7, 2010 6:14 PM. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. Devices FilterMax Multi-Mode Microplate Readers. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Transfluor Technology. Then, 50 µL of a stop solution were applied to terminate the reaction. Description: The FilterMax™ F3 features absorbance (visible),. 0´ 104 8. SpectraMax M-series Microplate readers. Luminescence was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). 020 fmol 0. Measurement of Caspase 3/7 Activity. 5. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Caspase-3 Activity. The fluorescent signals were measured at 10 minutes and one hour after the Stoplight Red substrateFilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) 12. Cytokines Assay. LDH cytotoxicity assaySome of new FilterMax F5 Multi-Mode Microplate readers are shipped with ARP USB to serial port adaptor (Manufacturer No: 572213, ARP No: 465338, 1 X USB, 1 X serial DB9/m, blue). 2. Mazumder, in Laser Additive Manufacturing, 2017 1. 00. 3 Pages. ” 3. 561. Page 24: Synergy™ Ht Multi-Mode Microplate Reader Internal plate shaking is also supported. Page 1 SpectraMax® M3 SpectraMax® M4 SpectraMax® M5 SpectraMax® M5e Multi-Mode Microplate Readers User Guide 0112-0115 G October 2018. To read the absorbance, a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA 95134, USA) with a wavelength of 595 nm was used. The results were calculated using a malondialdehyde (MDA) curve and expressed as nmol MDA/mg protein. 00 USD. 00 USD. Other recommended products. Instrument Configuration: Assay Technology Adapta® & Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET only) (TR-FRET only) HTRF Certification LanthaScreen Certification Key. A 1:1 binding ratio Hill model was used to fit the binding interaction data. The following document is intended to demonstrate setup of this instrument. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. MetaFluor Software. Open the catalog to page 1. We have 2 Molecular Devices FilterMax F5 manuals available for free PDF download:. Multi-Mode Microplate Reader. MetaFluor Software. F3/F5 Multi-Mode Microplate Readers. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. com 8. 2 Pages. How to Get Multi-Day Kinetic Data. 50/50. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. Devices FilterMax Multi-Mode Microplate Readers. » FilterMax™ F5 Multi-Mode Microplate Reader » SpectraMax L Microplate Luminometer ATP + Luciferin + 0 2 Oxyluciferin + AMP + PPi + CO 2 + Light Mg2+ Luciferase Benefits • Sensitive, reliable detection of mycoplasma contamination. ” e. Trinean Dropsense 96 Polychromatic. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. To contact technical support by phone in the U. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. . (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. 2 Pages. You can also contact Technical Support by email. After the mixture was cooled, TBARS was measured at 532 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Reader, Molecular Devices). Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. the luminescence reading was taken on a FilterMax F5 Multi-Mode. Caspase-3/7 activity, an indicator of apoptosis, was measured by a luminescence assay using caspase-Glo. 7. Learn how to set up protocols, customize reports, export results, and more with this PDF document. 0´ 105 dsD NA (p g/ wel ) R F U • Gemini™ EM/XPS Fluorescence Microplate Readers A B C Optimal data qualityGeneral Laboratory Instruments. Installation Qualification (IQ)Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). The 50% inhibitory concentration (IC 50) values were determined using the nonlinear regression curve fit analysis of Prism 9. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. ABS Test results. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. • DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. 00 USD. Published Dec 16, 2020 | Updated Dec 16, 2020. 3 Pages. 3 Pages. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. MetaMorph NX. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter incubation for 6 minutes, the mixture was read at 340 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Readers, Molecular Devices). Measurements of Caspase-3/7 Activity. The present study found competitive inhibition of hMAO-A by ILG along with a similar mode of action against hMAO-B as in rMAO-B. Location: San Diego, California, UNITED STATES Member Since: 17th September, 2003 (20 years ago) Current Listings: 666 Shipping Policy: Freight Shipping and Handling: For all orders, Name/Company Name, Address, and Contact Phone Number are required. 100 fmol 384-well (75 µL) 0. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. The ucs load command creates a backup of. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and. 2. Multi-Mode Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Instrument Configuration: Assay Technology Adapta® & F3/F5 Multi-Mode Microplate Readers. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. By category. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. MDA is one of the final products of LPO. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 2 mm) was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 15 min over 16 h incubation time. Serum concentrations of CAF were determined according to manufacturer guidelines in duplicate via enzyme-linked immunosorbent assay (Glory Science Co. 003 fmol 0. Our SpectraTest® ABS2, FL1, LM1, and Multi-Mode Validation Packages provide automated,A delightfully easy to use multimode reader. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Then 100 μL of 2 mol L −1 sulphuric acid was added to stop the enzymatic reaction and the absorbance values were read at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#:. 3 Pages. 00. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. Discover Precision and Reliability with Our Featured General Laboratory Instruments - Thermo Forma 3110 Water Jacketed CO2 Incubator, Thermo ULT185-5-V -80 Freezer, Filtermax F5 Multimode Microplate Reader, Spectramax M5e, Thermo Napco CO2 Incubator, and XS 64 Balance. MetaFluor Software. – ™FilterMax F5 Multi-Mode Microplate Reader (cat. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to. FLIPR Membrane Potential Assay Kits. 00 USD. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Add to my manuals. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Luminescence (glow) Fluorescence polarization. The VICTOR Nivo multimode microplate reader is a high-performance filter-based plate reader equipped with all major detection technologies – Alpha, luminescence, etc. SpectraMax iD5. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium& FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Multi-mode Microplate Readers. CloneMatrix. ScanLater Western Blot Detection System. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. For WST-1–based assay, 10 μL ready-to-use WST-1 reagent was added to each well and incubated for 3 hours at 37°C, and absorbance at 450 nm was measured. Multi-mode Microplate Readers. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. 3 Pages. The results are expressed as cell viabilities (%) based on the control condition. Luminescence was recorded with a luminometer (Filter Max F5 Multi-Mode Microplate reader) with SoftMax Pro Easy software, and the NADH/NAD + ratio was calculated by comparing the relative light units. Internal controls were. DMSO was used to dissolve the compounds and was included in the microplate at 0. Transfluor Technology. 3 Pages. A blank sample was prepared by mixing. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia. After adding 100 μl of new ice-cold PBS to the cells, fluorescent intensity was immediately determined using the fluorescent plate reader (FilterMax F3&F5 Multi-Mode Microplate Reader, Molecular Devices). ScanLater Western Blot Detection System. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Published Dec 16, 2020 | Updated Dec 16, 2020. This can increase throughput and efficiency, thus saving time and space in the lab. 100 fmol 1536-well (8 µL) 0. If using a multi-functional reader, please be sure to check the sensitivity of the device using the MycoAlert™ Assay Control Set. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. RNA extraction and reverse transcriptase–quantitative PCR. Maximum haemolysis was obtained using ultrapure distilled water, and untreated erythrocytes were used as a negative control [14]. SpectraMax Paradigm Multi-Mode Microplate Reader. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. All ELISA kits were read using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices; San Jose, CA, USA), according to the kit guidelines. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. 5. Vertical Jump. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Products : General, FilterMax F5 reader. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. IMAP assays are based on binding of. performs within its specifications. Homogeneous Time-Resolved Fluorescence (HTRF) . FilterMax F3/F5 Multi- Mode Microplate Readers · FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: of 6 /6. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. Protein contamination can be measured at 280 nm. com Check our website for a current listing SpectraMax i3/i3x Multi-Mode Microplate Reader SpectraMax Paradigm Multi-Mode Microplate Reader MOLECULAR DEVICES The trademarks used herein are the. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry. Approximately 50 µl of these. Turn on the power switch on the back of the instrument. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. In the list below the slide number 21, select the positions where the emission filters are installedAfter 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). FilterMax F5 Multi-Mode Microplate Reader. Place plate in the GloMax® Instrument and read. Transfluor Technology. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers; SpectraMax QuickDrop Micro-Volume Spectrophotometer; EMax Plus Microplate. The OD value of each tube (230 μL) was measured in a 96-well microplate at 405 nm using a. Cover plate with gas-permeable membrane and put in incubator. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. 40266 Email: drugdiscoverytech@lifetech. Select the protocol “Concentration_pico_green_v3. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help save time and reduce common errors in the lab—including automated. microplate reader. 11 mg/mL in PBS, 20 h incubation) using the FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices (Sunnyvale, CA, USA), at 570 nm . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Page 4 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 5. By category. FilterMax F5 Multi-Mode Microplate Reader. 5. 3 Pages. Delete from my manuals. #ID5-STD), equipped with the following: Enhanced TRF Module (Molecular Devices cat. ~ Bio-Tek ELx808 Microplate Reader $2,795. Neurotransmitter Transporter Uptake Assay Kit. Safety Information. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. The amount of colorimetric product was continuously monitored for 120 min. 4 Pages. $3,000 USD. 3 Pages. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Molecular Devices, LLC Reading Modes: Endpoint, Kinetic, Linear Scan, Area Scan; Optical System: LEDs, Deuterium Lamp; Wavelength Range: 230 to 750 nm;. 3. --> Life sciences. Z’-LYTE® Compatible Microplate Reader Documentation Version No. Optical densities (OD) from ELISA assays were measured with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). J. d. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available)Find used microplate readers on Machinio to perform a wide range of assays including ELISAs, cell viability assays, nucleic acid quantification, and more. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. , Sunnyvale, CA, USA). 3 Pages. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease. MetaMorph NX. MIA PaCa-2 cells were cultured in 96-well plates with 1000 cells/well. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Caspase 3/7 activity was performed as previously described [8,9,10,20]. BMG Labtech - THERMOstar. SoftMax Pro Software, data can be visualized as gray scale, kinetic plots or reaction rates. . The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. After 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). 1% (v/v) formic acid, was adopted for lipid separation: 0–2 min. After 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). 2. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). CloneMatrix. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The absorbance values of the results were expressed as optical density (OD) as the mean ± standard deviation. Z’-LYTE® Compatible Microplate Reader Documentation Version No. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). 2. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Each condition was performed in. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. In the case of cocultures, donor and recipient. CloneMatrix. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). 4 Pages. • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Important things to consider when purchasing a multimode microplate reader. Click “Eject Plate Carrier. 0. Turn on the power to the FilterMax F5. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 405 was measured in a FilterMax™ F5 Multi-Mode Microplate Reader. Cell growth and viability were assessed using the CCK-8 proliferation assay. Multi-Mode Reader Comparison Table; Single Mode Readers. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. MetaMorph NX. Fluorescence was measured with excitation and emission wavelengths of 530 and 590 nm, respectively, on a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. A FilterMax F5 multi-mode microplate reader (Molecular Devices, San Jose, USA) was used to determine the absorbance (OD 450nm -OD 570nm). 2. Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual. SoftMax Pro GxP Compliance Software. The solution was incubated for 15 min and fluorescence measured on a SpectraMax microplate reader (Molecular Devices) with an. searchPolymerization and polymerization kinetics were monitored in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA) using a FilterMax F5 Multi-Mode Microplate Reader. 5 million in 2019 and is projected to reach US$ 704. Topic. F3 F5 F3-SC F5-SC F3 F5. SpectraMax Gemini XPS/EM Microplate Readers. Unsuccessful migration. FilterMax F3 / FilterMax F5: Inquire: SpectraMax iD3 and iD5: SpectraMax M: Price: Description: Unlimited breadth of user-upgradeable application modules expands research capabilities. 8 Pages. The instrument performs an initialization procedure that moves the optics and the microplate drawer to home positions. The data were analyzed using Multi-Mode Analysis software and normalized to fluorescence in the vehicle-treated cells (% of control). The results are expressed as cell viabilities (%) based on the control condition. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. 3 Pages. Products : General, FilterMax F5 reader Please click on the links below to view the Microplate Reader Comparison Tables. The. The door opens, revealing the transport lock that fastens the microplate drawer to the internal frame of the instrument. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seF3/F5 Multi-Mode Microplate Readers.